ELISA kits for the detection of BHV-1 gB and gE antibodies in serum and milk from bovine species for the Department of Agriculture, Food and the Marine

Description

The Department of Agriculture, Food and the Marine requires an ELISA system capable of identifying antibodies to BHV-1 virus in bovine serum and milk samples. One of the ELISA kits should be able to identify the antibodies to glycoprotein B in bovine serum. This ELISA will detect antibody responses as a result of both infection and vaccination including the gE deleted vaccine. It will be referred to as “the gB ELISA kit” A second ELISA kit will detect antibodies to the gE antigen of BHV-1 in individual bovine serum and in milk. The presence of antibodies to gE indicates exposure to field strains of BHV-1 and/or vaccines containing gE antigen. It will be referred to as “the gE ELISA kit”. Lot 1: The Department of Agriculture, Food and the Marine requires an ELISA system capable of identifying antibodies to BHV-1 virus in bovine serum and milk samples. One of the ELISA kits should be able to identify the antibodies to glycoprotein B in bovine serum. This ELISA will detect antibody responses as a result of both infection and vaccination including the gE deleted vaccine. It will be referred to as “the gB ELISA kit” A second ELISA kit will detect antibodies to the gE antigen of BHV-1 in individual bovine serum and in milk. The presence of antibodies to gE indicates exposure to field strains of BHV-1 and/or vaccines containing gE antigen. It will be referred to as “the gE ELISA kit”. Lot 1: The Department of Agriculture, Food and the Marine requires an ELISA system capable of identifying antibodies to BHV-1 virus in bovine serum and milk samples. One of the ELISA kits should be able to identify the antibodies to glycoprotein B in bovine serum. This ELISA will detect antibody responses as a result of both infection and vaccination including the gE deleted vaccine. It will be referred to as “the gB ELISA kit” A second ELISA kit will detect antibodies to the gE antigen of BHV-1 in individual bovine serum and in milk. The presence of antibodies to gE indicates exposure to field strains of BHV-1 and/or vaccines containing gE antigen. It will be referred to as “the gE ELISA kit”.